Directed evolution of unspecific peroxygenase in organic solvents
نویسندگان
چکیده
منابع مشابه
Directed evolution of unspecific peroxygenase from Agrocybe aegerita.
Unspecific peroxygenase (UPO) represents a new type of heme-thiolate enzyme with self-sufficient mono(per)oxygenase activity and many potential applications in organic synthesis. With a view to taking advantage of these properties, we subjected the Agrocybe aegerita UPO1-encoding gene to directed evolution in Saccharomyces cerevisiae. To promote functional expression, several different signal p...
متن کاملSurface-directed channels filled with organic solvents.
Although channels of microfluidic devices are normally pipes, some groups have recently studied surface-directed channels. These channels were created between two stacked glass slides that were physically flat but chemically patterned. The working fluids used for such channels were mostly water solutions, though organic solvents are important in some applications such as organic synthesis. In t...
متن کاملTherapy of unspecific tinnitus without organic cause
INTRODUCTION There is a variety of medical and non-medical therapies in practice, which were not evaluated regarding its effectiveness by any systematic evidence oriented investigation. A number of therapies of medical and non-medical type try to treat the different types of tinnitus. The evidence in the scientific literature also had to be cleared in the field of diagnosis and classification a...
متن کاملA thermostable variant of fructose bisphosphate aldolase constructed by directed evolution also shows increased stability in organic solvents.
Thermostable variants of the Class II fructose bisphosphate aldolase have been isolated following four rounds of directed evolution using DNA shuffling of the fda genes from Escherichia coli and Edwardsiella ictaluri. Variants from all four generations of evolution have been purified and characterized. The variants show increased thermostability with no loss of catalytic function at room temper...
متن کاملChemiluminescence Properties of Peroxyoxalate System in Organic Solvents
Peroxyoxalate system includes an oxalate ester, peroxide and a fluorescer, The energy resulted from the reaction of oxalate ester and peroxide is transferred to fluorescer and cause it to fluorsc. The effect of reactants chemical structure, their concentration, solvent media (polarity and viscosity) and the temperature, on the intensity of the illuminated light with respect to time have bee...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 2021
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.27810